86 CULTURE OF PIG EMBRYOS BEFORE CRYOPRESERVATION
نویسندگان
چکیده
منابع مشابه
I-11: Cryopreservation of Canine Embryos
Background: The assisted reproductive techniques (ARTs) such as in vitro fertilization, embryo transfer and cryopreservation of gametes have considerably contributed to the development of biomedical sciences in addition to improved breeding in domestic animals and infertility treatment in humans. However, ARTs used in canine species have strictly limited utility when compared with other mammali...
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To determine the best and simplest method for cryopreservation of pig hepatocytes, we compared immediate cryopreservation with cryopreservation after short-term culture. Suckling pig hepatocytes were isolated by a modified 2-step in situ collagenase perfusion method, suspended in serum-free medium, and preserved for 10 da by two cryopreservation methods. Serial measurements were made of cell vi...
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The cryopreservation of mammalian oocytes and embryos has application to preserve genetic diversity and for in vitro fertilization protocols in humans. To date, cryopreservation techniques have been used in a variety of species, including rabbits [1, 2], cattle [3, 4], horses [5, 6], cats [7, 8], mice [9, 10] and ferrets [11], among others. However, because of the biological uniqueness of porci...
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This study reports that common carp (Cyprinus carpio) embryos were successfully frozen and preserved by vitrification. The modified Haga solution was tested by using two different cryoprotectants and three different concentrations at 4°C to obtain appropriate vitrified solutions ((dimethylsulfoxide 5, 10, 15% (DMSO) and glycerol 5, 10, 15% (gly)) in the study. The hatching rates of embryos exp...
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A total of 410 goat embryos were divided at random into 9 groups. Cryoprotectants (glycerol, ethylene glycol or dimethylsulfoxide) were added by a 3-step procedure using increasing concentrations of cryoprotectant (0.5 M; 1 M; 1.5 M) in PBS at 10 min intervals. After freezing and thawing, each cryoprotectant was removed by 3 methods: the classic 3-step procedure (cryoprotectant 1 M-10 min; 0.5 ...
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ژورنال
عنوان ژورنال: Reproduction, Fertility and Development
سال: 2005
ISSN: 1031-3613
DOI: 10.1071/rdv17n2ab86